Cincinnati Children's Hospital Medical Center - Research Careers

Research Jobs
at Cincinnati Children's Hospital Medical Center

Full Job Title: RESEARCH ASSOCIATE - Systems Immunology

Job Number: 95133

Location: Cincinnati, OH

Date Posted: 10-13-2017


DEPARTMENT:Cincinnati Children's Research Foundation, CCHMC Affiliates

SUPERVISOR'S TITLE: Principal Investigator



PURPOSE OF POSITION: To conduct independent and directed scientific studies and research in support of a Principal Investigator's research for a Medical Center.
The Roskin Lab at Cincinnati Children's Hospital and Medical Center is seeking a postdoctoral Research Associate interested in studying the adaptive immune system by massively parallel sequencing of B and T cell receptors elicited under various immune conditions.
The Roskin Lab is a new lab that focuses on combining computational and molecular biology methods to better understand the immune system. Affiliated with both the Immunobiology and Biomedical Informatics Divisions, the goal of the Roskin Lab is to study and catalog changes in the immune receptor repertoire and link those changes to immunogen exposure or autoimmunity/immunodeficiency status.
While the focus of the lab is human immunology, the group is also interested in applying these methods to study model organisms with collaborators.

Key Responsibilities
·Conduct research:
-Production of high-throughput sequencing libraries by RT-PCR, amplicon PCR, and molecular barcoding or single-cell droplet amplification;
-Isolation of immune cell types by fluorescent-activated cell sorting (FACS);
-Development, validation, and troubleshooting of new or improved sequencing library production methods;
-Work with computational immunology researchers on analysis strategies; Offer suggestions regarding modifications to procedures and protocols in collaboration with lab members;
-Review literature on an ongoing basis to remain current with new procedures and related research;
-Follow good laboratory practices, including maintenance of accurate lab notebooks;
-Discuss relevant insights about experimental results with Director and provide useful recommendations and analysis;
-Troubleshoot and correct erroneous results or problems with laboratory equipment. Maintain and repair equipment; monitor proper use by personnel;
-Maintain optimal protocol and assay accuracy by conducting regular quality and reproducibility checks;
-Demonstrate performance at work, consistency in carrying out instructions, accuracy & attention to detail, achieving expected results & continuous improvement;

·Publish: Draft, write and edit scientific reports, papers, journal articles and abstracts. Present at local, national and international scientific meetings.

·Teamwork:Train and supervise the work of other laboratory personnel as appropriate. Maintain an integral role in the laboratory's meetings, presentations and publications. Present information on research and laboratory work to others at laboratory meetings, journal clubs and seminars. Demonstrate attendance and punctuality, ability to work independently & display initiative and dedication.

·Financial Management: Assist in preparing, writing and maintaining fiscal budget. Monitor expenditures and prepare capital expenditure requests. Prepare, approve and initiate check requests; track funding, maintain accurate balance and resolve any discrepancies.

·Leadership: May act as a mentor to more junior staff, serve as the contact for resolution of complex issues, provide education, guidance and work direction. May provide input and feedback to managers on hiring decisions, professional development and performance management.

·PhD in immunology, molecular biology, biochemistry, or a related discipline is required
·Experience developing assays based on next generation sequencing
·Experience in analysis of sequencing data, programming ability, and familiarity with statistical software packages a significant plus
·Experience in single-cell genomics, functional genomics, or immunology a preferred
·Experience and familiarity working safely with blood
·Strong organizational and communication skills.
·Detail-oriented and ability to work independently.

REQUIRED SKILLS: Excellent written, verbal and interpersonal communication skills. Demonstrate attention to detail, strong organizational skills, supervisory ability and an innovative approach to experimental design. Computer literate and working knowledge of software applications (word processing, presentation, spreadsheet and database software). Analytical ability sufficient to compare data; mathematical ability sufficient to perform algebraic calculations and statistical analyses. Ability to synthesize information and to create and deal with new situations by applying past experiences. Expressed willingness to teach others and share results. Capable of meeting time demands necessary to execute experiments. Ability to express results in the form of oral and written presentations suitable for submission to the peer-reviewed literature. Careful bookkeeping skills. Expertise in technical execution and precision measurements. Presentation of experimental results in a form suitable for extramural funding applications.

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